高效液相色谱法同时测定车前子配方颗粒中京尼平苷酸、咖啡酸、毛蕊花糖苷和异毛蕊花糖苷的含量

田伟, 甄亚钦, 范帅帅, 窦玉红, 陈钟, 牛丽颖

中国药学杂志 ›› 2018, Vol. 53 ›› Issue (2) : 140-144.

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中国药学杂志 ›› 2018, Vol. 53 ›› Issue (2) : 140-144. DOI: 10.11669/cpj.2018.02.013
论 著

高效液相色谱法同时测定车前子配方颗粒中京尼平苷酸、咖啡酸、毛蕊花糖苷和异毛蕊花糖苷的含量

  • 田伟1,2,3, 甄亚钦1,2,3, 范帅帅1, 窦玉红1,2,3, 陈钟2,3,4, 牛丽颖1,2,3*
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Simultaneous Determination of Geniposidic Acid, Caffeic Acid, Acteoside and Isoacteoside in Plantaginis Semen Formula Granules by HPLC

  • TIAN Wei1,2,3, ZHEN Ya-qin1,2,3, FAN Shuai-shuai1, DOU Yu-hong1,2,3, CHEN Zhong2,3,4, NIU Li-ying1,2,3*
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摘要

目的 建立车前子配方颗粒中京尼平苷酸、咖啡酸、毛蕊花糖苷和异毛蕊花糖苷的含量测定方法,为其质量标准的研究提供依据。方法 采用Kinetex C18色谱柱(2.1 mm×100 mm,2.6 μm),以乙腈-0.5%乙酸溶液为流动相,梯度洗脱,流速为0.3 mL·min-1,检测波长分别为:京尼平苷酸239 nm,咖啡酸325 nm,毛蕊花糖苷和异毛蕊花糖苷330 nm;柱温30 ℃。结果 京尼平苷酸、咖啡酸、毛蕊花糖苷和异毛蕊花糖苷的进样量分别在0.292 1~2.921 μg(r=0.999 9),0.003 4~0.033 6 μg(r=0.999 8),0.047 6~0.476 μg(r=0.999 8),0.102 7~1.027 μg(r=0.999 9)内与峰面积呈良好的线性关系,平均回收率分别为99.32%,98.62%,98.23%和98.51%,RSD分别为1.47%,1.36%,1.62%和1.53%。结论 该方法简便、准确,分离效果好,可为车前子配方颗粒的质量控制提供方法参考。

Abstract

OBJECTIVE To establish an HPLC method to simultaneously determine the contents of geniposidic acid, caffeic acid, acteoside and isoacteoside in Plantaginis Semen formula granules, in order to provide basis for studying its quality standards. METHODS An HPLC method was developed. Kinetex C18 column (2.1 mm×100 mm,2.6 μm)was used and eluted with mobile phase of 0.5% acetic acid-acetonitrile at the flow rate of 0.3 mL·min-1. The wavelength was set at 239 nm for geniposidic acid, 325 nm for caffeic acid, and 330 nm for acteoside and isoacteoside. The column temperature was maintained at 30 ℃. RESULTS The good linear relationships between the concentration and peak area were in the range of 0.292 1-2.92 1 μg for geniposidic acid, 0.003 4-0.033 6 μg for caffeic acid, 0.047 6-0.476 μg for acteoside and 0.102 7-1.027 μg for isoacteoside (r≥0.999 8), respectively. The average recoveries were 99.32%, 98.62%, 98.23% and 98.51% with RSDs of 1.47%, 1.36%, 1.62% and 1.53%, respectively. CONCLUSION The method is simple, feasible and reproducible and can be used for the quality control of Plantaginis Semen formula granules.

关键词

车前子配方颗粒 / 京尼平苷酸 / 咖啡酸 / 毛蕊花糖苷 / 异毛蕊花糖苷 / 含量测定

Key words

Plantaginis Semen formula granule / geniposidic acid / caffeic acid / acteoside / isoacteoside / content determination

引用本文

导出引用
田伟, 甄亚钦, 范帅帅, 窦玉红, 陈钟, 牛丽颖. 高效液相色谱法同时测定车前子配方颗粒中京尼平苷酸、咖啡酸、毛蕊花糖苷和异毛蕊花糖苷的含量[J]. 中国药学杂志, 2018, 53(2): 140-144 https://doi.org/10.11669/cpj.2018.02.013
TIAN Wei, ZHEN Ya-qin, FAN Shuai-shuai, DOU Yu-hong, CHEN Zhong, NIU Li-ying. Simultaneous Determination of Geniposidic Acid, Caffeic Acid, Acteoside and Isoacteoside in Plantaginis Semen Formula Granules by HPLC[J]. Chinese Pharmaceutical Journal, 2018, 53(2): 140-144 https://doi.org/10.11669/cpj.2018.02.013
中图分类号: R284   

参考文献

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基金

河北省中医药管理局科研计划项目资助(2017015)
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